A rapid, sensitive protein assay for the accurate analysis of protein concentrations

rapidly at room temperature (RT) to provide ready-to-read results within 5 minutes without the need for incubation at elevated temperatures (Figure 1). Similar to the BCA assay, the Pierce Rapid Gold BCA Protein Assay combines the well-known reduction of Cu2+ to Cu+ by proteins in an alkaline medium (biuret reaction) with highly sensitive and selective colorimetric detection by a new copper chelator. The amount of Cu2+ reduced is proportional to the amount of protein present in the solution. The selective copper chelator forms an orange-gold–colored complex that strongly absorbs light at 480 nm.